Journal: Journal of the Endocrine Society

Article Title: Gene Misexpression in a Smoc2 +ve/ Sox2 -Low Population in Juvenile Prop1 -Mutant Pituitary Gland

doi: 10.1210/jendso/bvae146

Figure Lengend Snippet: Single-cell sequencing of wild-type and Prop1 -mutant P4 female pituitary cells. (A) UMAP of integrated wild-type and mutant cells. The mutant lacks cells from certain cell populations (detailed below). (B) Computational clustering and assignment of likely identity (see Supplementary Fig. S1 ). (C) Difference in cell-type proportions in Prop1 -mutant mice. Prop1 -dependent cell types such as somatotropes, thyrotropes, and proliferating Pou1f1 -cells are depleted in the Prop1 -mutant as expected. As a result, gonadotropes and corticotropes are proportionally enriched. (D) Cell cycle phase scoring, identifying proliferating stem cells and Pou1f1 +ve cells. (E) Absence of Pou1f1 expression in the Prop1 -mutant sample. (F-I) Expression of Sox2 and other markers that differentiate the cleft and parenchymal stem cells.

Article Snippet: TaqMan Probe mouse assays (Catalog No.: 4331182): Lhb mm00656868_g1; Nr5a1 mm00446826_m1; Pou1f1 mm00476852_m1, Smoc2 mm00491553_m1; Sox21 mm00844350_s1; Sox2 mm03053810_s1; Hprt mm03024075_m1.

Techniques: Sequencing, Mutagenesis, Expressing

Journal: Journal of the Endocrine Society

Article Title: Gene Misexpression in a Smoc2 +ve/ Sox2 -Low Population in Juvenile Prop1 -Mutant Pituitary Gland

doi: 10.1210/jendso/bvae146

Figure Lengend Snippet: Detection of a transient juvenile Sox2 -low cell state in mouse pituitary. (A) Violin plot of Sox2 expression across calculated clusters. Cluster 6 cells express Sox2 , albeit lower than some other clusters. (B, C) Cluster 6 cells uniquely express Smoc2 . (D) RNA velocity trajectory analysis setting the cleft Sox2 +ve stem cells as the root suggests stem cells pass through the Smoc2 +ve cluster 6 as they differentiate into endocrine cells. (E) qPCR for Smoc2 from control pituitary glands at young ages show highest expression at P3. (F) RNAscope detection of diffuse Smoc2 transcripts and Slc16a2 in cleft cells in female P7 mouse pituitary.

Article Snippet: TaqMan Probe mouse assays (Catalog No.: 4331182): Lhb mm00656868_g1; Nr5a1 mm00446826_m1; Pou1f1 mm00476852_m1, Smoc2 mm00491553_m1; Sox21 mm00844350_s1; Sox2 mm03053810_s1; Hprt mm03024075_m1.

Techniques: Expressing, Control, RNAscope

Journal: Journal of the Endocrine Society

Article Title: Gene Misexpression in a Smoc2 +ve/ Sox2 -Low Population in Juvenile Prop1 -Mutant Pituitary Gland

doi: 10.1210/jendso/bvae146

Figure Lengend Snippet: Ectopic expression of Sox21 in the Smoc2 -expressing population in Prop1 df/df mice. (A-B) Sox21 is upregulated in Prop1 -mutants in cluster 6, the Sox2/Smoc2 population. (C) Sox21 upregulation occurs in Prop1 df/df mutants but not Pou1f1 dw/dw mutants, another model of pituitary hormone deficiency. (D) SOX21 ectopic expression in Prop1 df/df pituitary glands in P3 and P7 mice.

Article Snippet: TaqMan Probe mouse assays (Catalog No.: 4331182): Lhb mm00656868_g1; Nr5a1 mm00446826_m1; Pou1f1 mm00476852_m1, Smoc2 mm00491553_m1; Sox21 mm00844350_s1; Sox2 mm03053810_s1; Hprt mm03024075_m1.

Techniques: Expressing

Journal: Journal of the Endocrine Society

Article Title: Gene Misexpression in a Smoc2 +ve/ Sox2 -Low Population in Juvenile Prop1 -Mutant Pituitary Gland

doi: 10.1210/jendso/bvae146

Figure Lengend Snippet: Pou3f4 overexpression in Prop1 -mutants occurs in Smoc2/Sox2 cells but does not appear to drive Sox21 overexpression. (A) Transcription factors such as Lef1 and Pou3f4 are also expressed specifically in the Smoc2 +ve population. (B) Average Pou3f4 expression per cell per cluster, split by genotype, is significantly increased in the Smoc2 -expressing cluster 6 in Prop1 -mutant cells. (C) P1 Pou3f4 -mutant mice have normal pituitary morphology and (D) Sox2 and Pou1f1 expression are normal. (E) Double deletion of Prop1 and Pou3f4 does not reverse Prop1 -mutant phenotypes such as Sox21 ectopic expression or hypoplasia and dysmorphology (Supplementary Fig. S2 ). For D-E, blue points represent male samples and orange points represent female samples.

Article Snippet: TaqMan Probe mouse assays (Catalog No.: 4331182): Lhb mm00656868_g1; Nr5a1 mm00446826_m1; Pou1f1 mm00476852_m1, Smoc2 mm00491553_m1; Sox21 mm00844350_s1; Sox2 mm03053810_s1; Hprt mm03024075_m1.

Techniques: Over Expression, Expressing, Mutagenesis

Journal: Cell Reports Medicine

Article Title: Outer radial glia promotes white matter regeneration after neonatal brain injury

doi: 10.1016/j.xcrm.2025.101986

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-Sox2 , Cell Signaling , Cat# 2748; RRID: AB_823640.

Techniques: Virus, Plasmid Preparation, Recombinant, RNAscope, Blocking Assay, Staining, Knock-Out, In Situ, Multiplex Assay, Sequencing, Software

Journal: Cell Reports Medicine

Article Title: Outer radial glia promotes white matter regeneration after neonatal brain injury

doi: 10.1016/j.xcrm.2025.101986

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-Sox2 , Santa Cruz , Cat# sc-365823; RRID: AB_10842165.

Techniques: Virus, Plasmid Preparation, Recombinant, RNAscope, Blocking Assay, Staining, Knock-Out, In Situ, Multiplex Assay, Sequencing, Software

Journal: eLife

Article Title: Distinct mesoderm migration phenotypes in extra-embryonic and embryonic regions of the early mouse embryo

doi: 10.7554/eLife.42434

Figure Lengend Snippet: ( a, b ) Embryonic mesoderm explants from ( a ) mTmG; Rhoa Δmesoderm , and ( b ) mTmG; Rac1 Δmesoderm Mid/Late Streak embryos cultured on fibronectin for ( a ) 48 hr and ( b ) 30 hr, stained for F-actin (Phalloidin, in red) and nuclei (DAPI, in blue). mG: membrane GFP, in green. (n=3 for Rac1 , 4 for Rhoa ; similar phenotype in 2/3 mutants for Rac1 and 3/4 mutants for Rhoa ). (Scale bars: 200 μm for a, and 50 μm for b). ( c ) Radial expansion from embryonic mesoderm explant of mTmG; Rhoa Δmesoderm embryo, after 48 hr of culture on fibronectin. Explants images were fitted inside their corresponding circle, and cropped in the form of cone from the center. (Scale bars: 100 μm). ( c ) Roundness quantification (mean ± SEM) in wild-type and Rhoa Δmesoderm mesoderm explants. (n=250 cells for wild-type, 272 for Rhoa Δmesoderm , p<0.0001). Data can be found in . ( d ) Z-projections of middle slices from two-photon stacks of wild-type (left) and Rac1 Δepiblast (right) embryos expressing mGFP in epiblast-derived ( Sox2 -Cre) cells. Anterior is to the left. Arrows point to extra-embryonic mesoderm. Dashes line the epiblast (Scale bars: 50 μm). ( e, f ) Whole-mount E8.5 ( e ) Rhoa Δmesoderm and ( f ) Rac1 Δmesoderm embryos stained for Pecam-1 (in yellow, n=2 for Rhoa , 8 for Rac1 ). Dashed lines mark the yolk sac. (Scale bars: 100 μm). P values were calculated using the Mann–Whitney–Wilcoxon. All mutants are compared to a wild-type littermate. 10.7554/eLife.42434.038 Figure 6—figure supplement 2—source data 1. Quantification of cell shape in mesoderm explants from wild-type and Rhoa Δmesoderm embryos.

Article Snippet: Genetic reagent ( Mus musculus ) , Sox2 -Cre , PMID: 12617844 ; The Jackson Laboratories , TJL: 008454 , .

Techniques: Cell Culture, Staining, Membrane, Expressing, Derivative Assay, MANN-WHITNEY

Journal: eLife

Article Title: Distinct mesoderm migration phenotypes in extra-embryonic and embryonic regions of the early mouse embryo

doi: 10.7554/eLife.42434

Figure Lengend Snippet:

Article Snippet: Genetic reagent ( Mus musculus ) , Sox2 -Cre , PMID: 12617844 ; The Jackson Laboratories , TJL: 008454 , .

Techniques: Mutagenesis, RNAscope

Journal: Cell reports

Article Title: A cell-type-specific atlas of the inner ear transcriptional response to acoustic trauma

doi: 10.1016/j.celrep.2021.109758

Figure Lengend Snippet:

Article Snippet: Mouse: Sox2-CreERT2 , The Jackson Laboratory , Stock No: 017593; RRID:IMSR_JAX:017593.

Techniques: Recombinant, Staining, RNAscope, Multiplex Assay, Sequencing, Software, Gene Expression

Journal: eLife

Article Title: Delineating the early transcriptional specification of the mammalian trachea and esophagus

doi: 10.7554/eLife.55526

Figure Lengend Snippet:

Article Snippet: Antibody , α-SOX2 (goat polyclonal) , Neuromics , Cat#: GT15098 , 1:250 for IF.

Techniques: Sequencing, RNAscope, MicroChIP Assay, DNA Extraction, RNA Extraction, Staining

Journal: eLife

Article Title: Decoding the activated stem cell phenotype of the neonatally maturing pituitary

doi: 10.7554/eLife.75742

Figure Lengend Snippet: ( A ) Differentially expressed gene (DEG)-based GSEA plots of indicated WNT-related hallmarks in neonatal versus adult stem cell clusters (SC1, SC2, and Prolif SC). Normalized enrichment score (NES), and p and FDR values are listed. ( B ) Heatmaps displaying scaled expression of several WNT ligand and receptor genes in adult and neonatal anterior pituitary (AP). ( C ) Left : Violin plots displaying mRNA expression level of indicated genes in SC1, SC2, MC, and Prolif SC of adult and neonatal AP. Right : Bar graphs displaying relative gene expression of indicated genes in neonatal AP, as determined by RT-qPCR (relative to expression in adult AP, set as 1 [dashed line]) (mean ± standard error of the mean [SEM]). Data points represent biological replicates ( n = 3; unpaired t -test). ( D ) Left : Projection on UMAP plot of selected WNT-associated genes’ expression in neonatal stem cell (SC1, SC2, and Prolif SC) and MC clusters, with indication of cell clusters. Right : RNAscope in situ hybridization analysis of neonatal pituitary for Sox2 (red), Lgr4 , Lgr6 , and Rspo3 (all green). Nuclei are stained with DAPI (blue). Arrowheads indicate the marginal zone (MZ) (scale bar, 100 μm).

Article Snippet: Differently labeled RNAscope probes (Advanced Cell Diagnostics) were used for mouse Sox2 (401041-C3), Rspo3 (483781-C2), Lgr4 (318321-C2), and Lgr6 (404961-C2).

Techniques: Expressing, Gene Expression, Quantitative RT-PCR, RNAscope, In Situ Hybridization, Staining

Journal: eLife

Article Title: Decoding the activated stem cell phenotype of the neonatally maturing pituitary

doi: 10.7554/eLife.75742

Figure Lengend Snippet:

Article Snippet: Differently labeled RNAscope probes (Advanced Cell Diagnostics) were used for mouse Sox2 (401041-C3), Rspo3 (483781-C2), Lgr4 (318321-C2), and Lgr6 (404961-C2).

Techniques: Isolation, Sequencing, RNAscope, Recombinant, Multiplex Assay, SYBR Green Assay, Software

Journal: bioRxiv

Article Title: Decoding the activated stem cell phenotype of the vividly maturing neonatal pituitary

doi: 10.1101/2022.01.18.476723

Figure Lengend Snippet: Neonatal pituitary stem cells show a pronounced WNT profile. (A) DEG-based GSEA plots of indicated WNT-related hallmarks in neonatal versus adult stem cell clusters (SC1, SC2, Prolif SC). Normalized enrichment score (NES), and P - and FDR-values are listed. (B) Heatmaps displaying scaled expression of several WNT ligand and receptor genes in adult and neonatal AP. (C) Left: Violin plots displaying mRNA expression level of indicated genes in SC1, SC2, MC and Prolif SC of adult and neonatal AP. Right: Bar graphs displaying relative gene expression of indicated genes in neonatal AP, as determined by RT-qPCR (relative to expression in adult AP, set as 1 (dashed line)) (mean ± SEM). Data points represent biological replicates. ** P ≤ 0.01. (D) Left: Projection on UMAP plot of selected WNT-associated genes’ expression in neonatal stem cell (SC1, SC2, Prolif SC) and MC clusters, with indication of cell clusters. Right: RNAscope in situ hybridization analysis of neonatal pituitary for Sox2 (red), Lgr4, Lgr6 and Rspo3 (all green). Nuclei are stained with DAPI (blue). Arrowheads indicate the marginal zone (MZ). (Scale bar, 100 μm). (E) Bar graph displaying relative gene expression level of indicated genes in neonatal AP-derived organoids (mean ± SEM). Data points represent biological replicates. (F) Organoid development from neonatal AP cells, cultured and exposed to WNT ligands as indicated (P0). Top: Representative brightfield pictures of organoid cultures. (Scale bar, 500 μm). Bottom: Bar graph showing number of organoids formed under conditions as indicated (mean ± SEM). Data points represent biological replicates. (G) Top: Immunofluorescence staining of Ki67 (red) in neonatal AP organoids formed under conditions as indicated (P0). Nuclei are stained with Hoechst33342 (blue). Arrowheads indicate Ki67 + cells. (Scale bar, 100 µm). Bottom: Bar graphs showing percentage of Ki67 + cells in organoids as indicated (relative to DMSO, set as 1 (dashed line)) (mean ± SEM). Data points represent individual organoids from 3 biological replicates. * P ≤ 0.05. (H) Top: Projection on UMAP plot of Rspo1 gene expression in neonatal stem cell (SC1, SC2, Prolif SC) and MC clusters, with indication of cell clusters. Bottom: Organoid development from neonatal AP cells formed in standard RSpECT medium (with RSPO1) or RSpECT medium in which RSPO1 was replaced with RSPO3 (P0). Representative brightfield images are shown. (Scale bar, 500 μm). (I) Organoid development from adult AP cells formed under conditions as indicated (P0). Top left: Representative brightfield pictures of organoid cultures. (Scale bar, 500 μm). Bottom left: Immunofluorescence staining of Ki67 (green) in adult AP organoids, formed under conditions as indicated. Nuclei are stained with Hoechst33342 (blue). (Scale bar, 100 µm). Middle: Bar graph showing percentage of Ki67 + cells in organoids as indicated (relative to PitOM, set as 1 (dashed line)) (mean ± SEM). Right: Violin plot showing diameter of organoids developed in conditions as indicated. Data points represent biological replicates. * P ≤ 0.05. (J) Left: UMAP plot of fetal human and neonatal mouse AP combined. Middle and right: UMAP plot of annotated cell clusters in human and mouse AP, respectively. Somato, somatotropes; Lacto, lactotropes; Cortico, corticotropes; Gonado, gonadotropes; Thyro, thyrotropes; Melano, melanotropes; SC1 and SC2, stem cell cluster 1 and 2; EC, endothelial cells; IC, immune cells; CT, connective tissue cells; PL, posterior lobe (pituicyte) cells; Gonado Prog, gonado progenitor cells; Gonado Prec, gonadotrope precursor cells; CC, cell cycle cells; RBC, red blood cells, Pro.PIT1, progenitor cells of PIT1 lineage; Pre.Gonado, precursor cells of gonadotropes. (K) Projection of Il6 gene expression on human fetal pituitary UMAP plot, with indication of cell clusters.

Article Snippet: Differently labeled RNAscope probes (Advanced Cell Diagnostics) were used for mouse Sox2 (401041-C3), Rspo3 (483781-C2), Lgr4 (318321-C2) and Lgr6 (404961- C2).

Techniques: Expressing, Quantitative RT-PCR, In Situ Hybridization, Staining, Derivative Assay, Cell Culture, Immunofluorescence

Journal: eLife

Article Title: Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells

doi: 10.7554/eLife.59142

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-SOX2 (Goat Polyclonal) , Immune Systems Ltd , GT15098, RRID: AB_2195800 , IF(1:200).

Techniques: Isolation, Sequencing, RNAscope, Positive Control, Negative Control, Recombinant, Staining, Software